Exploring transcriptional conservation between Ancylostoma caninum and Haemonchus contortus by oligonucleotide microarray and bioinformatic analyses

C Cantacessi; A Loukas; BE Campbell; J Mulvenna; EK Ong; W Zhong; PW Sternberg; D Otranto; RB Gasser

Journal article

Exploring transcriptional conservation between Ancylostoma caninum and Haemonchus contortus by oligonucleotide microarray and bioinformatic analyses

C Cantacessi, A Loukas, BE Campbell, J Mulvenna, EK Ong, W Zhong, PW Sternberg, D Otranto, RB Gasser

Molecular and Cellular Probes | Published : 2009

DOI: 10.1016/j.mcp.2008.09.004

Abstract

In this study, we identified, using an established oligonucleotide microarray platform for the parasitic nematode Haemonchus contortus, transcripts that are 'conserved' between serum-activated and non-activated L3s of Ancylostoma caninum (aL3 and L3, respectively) and H. contortus by cross-species hybridization (CSH) at high stringency and conducted extensive bioinformatic analyses of the cross-hybridizing expressed sequence tags (ESTs). The microarray analysis revealed significant differential hybridization between aL3 and L3 for 32 molecules from A. caninum, of which 29 were shown to have homologues/orthologues in the free-living nematode Caenorhabditis elegans and/or A. caninum and the ot..

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University of Melbourne Researchers

Robin B. Gasser Author

Related Projects (1)

Discovery of early developmental events in the transition to parasitism in the hookworm Ancylostoma caninum using genomic technologies

The national/community benefits are: (1) to develop a long-term, high quality scientific and technological program contributing to national ..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

This study was supported by the Australian Research Council (ARC) (DP0665230). The authors thank Bennett Datu for producing and providing RNA from A. caninum used in the present study. CC is the recipient of scholarships from the University of Melbourne. The Australian-American Fulbright Commission and Australian Academy of Science are gratefully acknowledged (RBG).